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CEM Corporation cem-c1 cell lines
Cem C1 Cell Lines, supplied by CEM Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cem-c1+cells/cem+c1+cells/pm40277564-104-5-5
Average 90 stars, based on 1 article reviews
cem-c1 cell lines - by Bioz Stars, 2026-10
90/100 stars

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Cell Culture:

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: To further confirm whether honokiol increases the sensitivity of DEXresistant CEM-C1 cells to dexamethasone, the DEX- resistant CEM-C1 cells and DEX-sensitive CEM-C7 cells were incubated with different concentrations of dexamethasone (0–400 μM) in combination with honokiol (1.5 μM) for 48 h. Our preliminary drug concentration screening results showed that the cell proliferation inhibition rate on the CEM-C1 and CEM-C7 cells treated with 1.5 μM honokiol was <4% (Fig. S1).

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Different concentrations of dexamethasone (50, 100, 200, 300, and 400 μM) with or without 1.5 μM HNK were added to the medium of CEM-C1 cells which are dexamethasone-resistant, whereas dexamethasone of 0.5, 1, 2, 3, and 4 μM with or without 1.5 μM HNK were added to the medium of CEM-C7 cells which are dexamethasone-sensitive.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 48 htreatment by dexamethasone with or without 1.5 μM honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Upon treatment with dexamethasone in combination with 1.5 μM of honokiol, the IC50 of CEM-C1 cells significantly decreased from 364.1 ± 29.5 to 126.2 ± 12.3 μM, and the reversal fold was 2.88, whereas the combination of dexamethasone and honokiol did not show significant impact on the IC50 of CEM-C7 cells as compared to that of CEM-C7 cells treated with dexamethasone alone (1.30 ± 0.13 versus 1.25 ± 0.14 μM; p > 0.05).


Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 24, 48, and 72 h-treatment by honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were cultured in DMEM supplemented with 10% of fetal bovine serum (FBS) at 37°C in a humidified atmosphere containing 5% of CO2.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were seeded at 3 × 105 cells/well in 6-well plates and pre-incubated for 24 h, and then treated with honokiol (0–20 μM) for 48 h. To extract the total protein, cells were washed with PBS, lysed in RIPA buffer for 30 min on ice.

Binding Assay:

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: To further confirm whether honokiol increases the sensitivity of DEXresistant CEM-C1 cells to dexamethasone, the DEX- resistant CEM-C1 cells and DEX-sensitive CEM-C7 cells were incubated with different concentrations of dexamethasone (0–400 μM) in combination with honokiol (1.5 μM) for 48 h. Our preliminary drug concentration screening results showed that the cell proliferation inhibition rate on the CEM-C1 and CEM-C7 cells treated with 1.5 μM honokiol was <4% (Fig. S1).

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Different concentrations of dexamethasone (50, 100, 200, 300, and 400 μM) with or without 1.5 μM HNK were added to the medium of CEM-C1 cells which are dexamethasone-resistant, whereas dexamethasone of 0.5, 1, 2, 3, and 4 μM with or without 1.5 μM HNK were added to the medium of CEM-C7 cells which are dexamethasone-sensitive.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 48 htreatment by dexamethasone with or without 1.5 μM honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Upon treatment with dexamethasone in combination with 1.5 μM of honokiol, the IC50 of CEM-C1 cells significantly decreased from 364.1 ± 29.5 to 126.2 ± 12.3 μM, and the reversal fold was 2.88, whereas the combination of dexamethasone and honokiol did not show significant impact on the IC50 of CEM-C7 cells as compared to that of CEM-C7 cells treated with dexamethasone alone (1.30 ± 0.13 versus 1.25 ± 0.14 μM; p > 0.05).


Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 24, 48, and 72 h-treatment by honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were cultured in DMEM supplemented with 10% of fetal bovine serum (FBS) at 37°C in a humidified atmosphere containing 5% of CO2.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were seeded at 3 × 105 cells/well in 6-well plates and pre-incubated for 24 h, and then treated with honokiol (0–20 μM) for 48 h. To extract the total protein, cells were washed with PBS, lysed in RIPA buffer for 30 min on ice.

Staining:

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: To further confirm whether honokiol increases the sensitivity of DEXresistant CEM-C1 cells to dexamethasone, the DEX- resistant CEM-C1 cells and DEX-sensitive CEM-C7 cells were incubated with different concentrations of dexamethasone (0–400 μM) in combination with honokiol (1.5 μM) for 48 h. Our preliminary drug concentration screening results showed that the cell proliferation inhibition rate on the CEM-C1 and CEM-C7 cells treated with 1.5 μM honokiol was <4% (Fig. S1).

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Different concentrations of dexamethasone (50, 100, 200, 300, and 400 μM) with or without 1.5 μM HNK were added to the medium of CEM-C1 cells which are dexamethasone-resistant, whereas dexamethasone of 0.5, 1, 2, 3, and 4 μM with or without 1.5 μM HNK were added to the medium of CEM-C7 cells which are dexamethasone-sensitive.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 48 htreatment by dexamethasone with or without 1.5 μM honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Upon treatment with dexamethasone in combination with 1.5 μM of honokiol, the IC50 of CEM-C1 cells significantly decreased from 364.1 ± 29.5 to 126.2 ± 12.3 μM, and the reversal fold was 2.88, whereas the combination of dexamethasone and honokiol did not show significant impact on the IC50 of CEM-C7 cells as compared to that of CEM-C7 cells treated with dexamethasone alone (1.30 ± 0.13 versus 1.25 ± 0.14 μM; p > 0.05).


Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 24, 48, and 72 h-treatment by honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were cultured in DMEM supplemented with 10% of fetal bovine serum (FBS) at 37°C in a humidified atmosphere containing 5% of CO2.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were seeded at 3 × 105 cells/well in 6-well plates and pre-incubated for 24 h, and then treated with honokiol (0–20 μM) for 48 h. To extract the total protein, cells were washed with PBS, lysed in RIPA buffer for 30 min on ice.

Incubation:

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: To further confirm whether honokiol increases the sensitivity of DEXresistant CEM-C1 cells to dexamethasone, the DEX- resistant CEM-C1 cells and DEX-sensitive CEM-C7 cells were incubated with different concentrations of dexamethasone (0–400 μM) in combination with honokiol (1.5 μM) for 48 h. Our preliminary drug concentration screening results showed that the cell proliferation inhibition rate on the CEM-C1 and CEM-C7 cells treated with 1.5 μM honokiol was <4% (Fig. S1).

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Different concentrations of dexamethasone (50, 100, 200, 300, and 400 μM) with or without 1.5 μM HNK were added to the medium of CEM-C1 cells which are dexamethasone-resistant, whereas dexamethasone of 0.5, 1, 2, 3, and 4 μM with or without 1.5 μM HNK were added to the medium of CEM-C7 cells which are dexamethasone-sensitive.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 48 htreatment by dexamethasone with or without 1.5 μM honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Upon treatment with dexamethasone in combination with 1.5 μM of honokiol, the IC50 of CEM-C1 cells significantly decreased from 364.1 ± 29.5 to 126.2 ± 12.3 μM, and the reversal fold was 2.88, whereas the combination of dexamethasone and honokiol did not show significant impact on the IC50 of CEM-C7 cells as compared to that of CEM-C7 cells treated with dexamethasone alone (1.30 ± 0.13 versus 1.25 ± 0.14 μM; p > 0.05).


Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 24, 48, and 72 h-treatment by honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were cultured in DMEM supplemented with 10% of fetal bovine serum (FBS) at 37°C in a humidified atmosphere containing 5% of CO2.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were seeded at 3 × 105 cells/well in 6-well plates and pre-incubated for 24 h, and then treated with honokiol (0–20 μM) for 48 h. To extract the total protein, cells were washed with PBS, lysed in RIPA buffer for 30 min on ice.

Fluorescence:

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: To further confirm whether honokiol increases the sensitivity of DEXresistant CEM-C1 cells to dexamethasone, the DEX- resistant CEM-C1 cells and DEX-sensitive CEM-C7 cells were incubated with different concentrations of dexamethasone (0–400 μM) in combination with honokiol (1.5 μM) for 48 h. Our preliminary drug concentration screening results showed that the cell proliferation inhibition rate on the CEM-C1 and CEM-C7 cells treated with 1.5 μM honokiol was <4% (Fig. S1).

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Different concentrations of dexamethasone (50, 100, 200, 300, and 400 μM) with or without 1.5 μM HNK were added to the medium of CEM-C1 cells which are dexamethasone-resistant, whereas dexamethasone of 0.5, 1, 2, 3, and 4 μM with or without 1.5 μM HNK were added to the medium of CEM-C7 cells which are dexamethasone-sensitive.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 48 htreatment by dexamethasone with or without 1.5 μM honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Upon treatment with dexamethasone in combination with 1.5 μM of honokiol, the IC50 of CEM-C1 cells significantly decreased from 364.1 ± 29.5 to 126.2 ± 12.3 μM, and the reversal fold was 2.88, whereas the combination of dexamethasone and honokiol did not show significant impact on the IC50 of CEM-C7 cells as compared to that of CEM-C7 cells treated with dexamethasone alone (1.30 ± 0.13 versus 1.25 ± 0.14 μM; p > 0.05).


Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 24, 48, and 72 h-treatment by honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were cultured in DMEM supplemented with 10% of fetal bovine serum (FBS) at 37°C in a humidified atmosphere containing 5% of CO2.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were seeded at 3 × 105 cells/well in 6-well plates and pre-incubated for 24 h, and then treated with honokiol (0–20 μM) for 48 h. To extract the total protein, cells were washed with PBS, lysed in RIPA buffer for 30 min on ice.

Concentration Assay:

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: To further confirm whether honokiol increases the sensitivity of DEXresistant CEM-C1 cells to dexamethasone, the DEX- resistant CEM-C1 cells and DEX-sensitive CEM-C7 cells were incubated with different concentrations of dexamethasone (0–400 μM) in combination with honokiol (1.5 μM) for 48 h. Our preliminary drug concentration screening results showed that the cell proliferation inhibition rate on the CEM-C1 and CEM-C7 cells treated with 1.5 μM honokiol was <4% (Fig. S1).

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Different concentrations of dexamethasone (50, 100, 200, 300, and 400 μM) with or without 1.5 μM HNK were added to the medium of CEM-C1 cells which are dexamethasone-resistant, whereas dexamethasone of 0.5, 1, 2, 3, and 4 μM with or without 1.5 μM HNK were added to the medium of CEM-C7 cells which are dexamethasone-sensitive.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 48 htreatment by dexamethasone with or without 1.5 μM honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Upon treatment with dexamethasone in combination with 1.5 μM of honokiol, the IC50 of CEM-C1 cells significantly decreased from 364.1 ± 29.5 to 126.2 ± 12.3 μM, and the reversal fold was 2.88, whereas the combination of dexamethasone and honokiol did not show significant impact on the IC50 of CEM-C7 cells as compared to that of CEM-C7 cells treated with dexamethasone alone (1.30 ± 0.13 versus 1.25 ± 0.14 μM; p > 0.05).


Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 24, 48, and 72 h-treatment by honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were cultured in DMEM supplemented with 10% of fetal bovine serum (FBS) at 37°C in a humidified atmosphere containing 5% of CO2.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were seeded at 3 × 105 cells/well in 6-well plates and pre-incubated for 24 h, and then treated with honokiol (0–20 μM) for 48 h. To extract the total protein, cells were washed with PBS, lysed in RIPA buffer for 30 min on ice.

Control:

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: To further confirm whether honokiol increases the sensitivity of DEXresistant CEM-C1 cells to dexamethasone, the DEX- resistant CEM-C1 cells and DEX-sensitive CEM-C7 cells were incubated with different concentrations of dexamethasone (0–400 μM) in combination with honokiol (1.5 μM) for 48 h. Our preliminary drug concentration screening results showed that the cell proliferation inhibition rate on the CEM-C1 and CEM-C7 cells treated with 1.5 μM honokiol was <4% (Fig. S1).

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Different concentrations of dexamethasone (50, 100, 200, 300, and 400 μM) with or without 1.5 μM HNK were added to the medium of CEM-C1 cells which are dexamethasone-resistant, whereas dexamethasone of 0.5, 1, 2, 3, and 4 μM with or without 1.5 μM HNK were added to the medium of CEM-C7 cells which are dexamethasone-sensitive.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 48 htreatment by dexamethasone with or without 1.5 μM honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Upon treatment with dexamethasone in combination with 1.5 μM of honokiol, the IC50 of CEM-C1 cells significantly decreased from 364.1 ± 29.5 to 126.2 ± 12.3 μM, and the reversal fold was 2.88, whereas the combination of dexamethasone and honokiol did not show significant impact on the IC50 of CEM-C7 cells as compared to that of CEM-C7 cells treated with dexamethasone alone (1.30 ± 0.13 versus 1.25 ± 0.14 μM; p > 0.05).


Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 24, 48, and 72 h-treatment by honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were cultured in DMEM supplemented with 10% of fetal bovine serum (FBS) at 37°C in a humidified atmosphere containing 5% of CO2.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were seeded at 3 × 105 cells/well in 6-well plates and pre-incubated for 24 h, and then treated with honokiol (0–20 μM) for 48 h. To extract the total protein, cells were washed with PBS, lysed in RIPA buffer for 30 min on ice.

Western Blot:

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: To further confirm whether honokiol increases the sensitivity of DEXresistant CEM-C1 cells to dexamethasone, the DEX- resistant CEM-C1 cells and DEX-sensitive CEM-C7 cells were incubated with different concentrations of dexamethasone (0–400 μM) in combination with honokiol (1.5 μM) for 48 h. Our preliminary drug concentration screening results showed that the cell proliferation inhibition rate on the CEM-C1 and CEM-C7 cells treated with 1.5 μM honokiol was <4% (Fig. S1).

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Different concentrations of dexamethasone (50, 100, 200, 300, and 400 μM) with or without 1.5 μM HNK were added to the medium of CEM-C1 cells which are dexamethasone-resistant, whereas dexamethasone of 0.5, 1, 2, 3, and 4 μM with or without 1.5 μM HNK were added to the medium of CEM-C7 cells which are dexamethasone-sensitive.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 48 htreatment by dexamethasone with or without 1.5 μM honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Upon treatment with dexamethasone in combination with 1.5 μM of honokiol, the IC50 of CEM-C1 cells significantly decreased from 364.1 ± 29.5 to 126.2 ± 12.3 μM, and the reversal fold was 2.88, whereas the combination of dexamethasone and honokiol did not show significant impact on the IC50 of CEM-C7 cells as compared to that of CEM-C7 cells treated with dexamethasone alone (1.30 ± 0.13 versus 1.25 ± 0.14 μM; p > 0.05).


Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 24, 48, and 72 h-treatment by honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were cultured in DMEM supplemented with 10% of fetal bovine serum (FBS) at 37°C in a humidified atmosphere containing 5% of CO2.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were seeded at 3 × 105 cells/well in 6-well plates and pre-incubated for 24 h, and then treated with honokiol (0–20 μM) for 48 h. To extract the total protein, cells were washed with PBS, lysed in RIPA buffer for 30 min on ice.

Quantitative RT-PCR:

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: To further confirm whether honokiol increases the sensitivity of DEXresistant CEM-C1 cells to dexamethasone, the DEX- resistant CEM-C1 cells and DEX-sensitive CEM-C7 cells were incubated with different concentrations of dexamethasone (0–400 μM) in combination with honokiol (1.5 μM) for 48 h. Our preliminary drug concentration screening results showed that the cell proliferation inhibition rate on the CEM-C1 and CEM-C7 cells treated with 1.5 μM honokiol was <4% (Fig. S1).

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Different concentrations of dexamethasone (50, 100, 200, 300, and 400 μM) with or without 1.5 μM HNK were added to the medium of CEM-C1 cells which are dexamethasone-resistant, whereas dexamethasone of 0.5, 1, 2, 3, and 4 μM with or without 1.5 μM HNK were added to the medium of CEM-C7 cells which are dexamethasone-sensitive.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 48 htreatment by dexamethasone with or without 1.5 μM honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Upon treatment with dexamethasone in combination with 1.5 μM of honokiol, the IC50 of CEM-C1 cells significantly decreased from 364.1 ± 29.5 to 126.2 ± 12.3 μM, and the reversal fold was 2.88, whereas the combination of dexamethasone and honokiol did not show significant impact on the IC50 of CEM-C7 cells as compared to that of CEM-C7 cells treated with dexamethasone alone (1.30 ± 0.13 versus 1.25 ± 0.14 μM; p > 0.05).


Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 24, 48, and 72 h-treatment by honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were cultured in DMEM supplemented with 10% of fetal bovine serum (FBS) at 37°C in a humidified atmosphere containing 5% of CO2.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were seeded at 3 × 105 cells/well in 6-well plates and pre-incubated for 24 h, and then treated with honokiol (0–20 μM) for 48 h. To extract the total protein, cells were washed with PBS, lysed in RIPA buffer for 30 min on ice.

Expressing:

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: To further confirm whether honokiol increases the sensitivity of DEXresistant CEM-C1 cells to dexamethasone, the DEX- resistant CEM-C1 cells and DEX-sensitive CEM-C7 cells were incubated with different concentrations of dexamethasone (0–400 μM) in combination with honokiol (1.5 μM) for 48 h. Our preliminary drug concentration screening results showed that the cell proliferation inhibition rate on the CEM-C1 and CEM-C7 cells treated with 1.5 μM honokiol was <4% (Fig. S1).

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Different concentrations of dexamethasone (50, 100, 200, 300, and 400 μM) with or without 1.5 μM HNK were added to the medium of CEM-C1 cells which are dexamethasone-resistant, whereas dexamethasone of 0.5, 1, 2, 3, and 4 μM with or without 1.5 μM HNK were added to the medium of CEM-C7 cells which are dexamethasone-sensitive.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 48 htreatment by dexamethasone with or without 1.5 μM honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Upon treatment with dexamethasone in combination with 1.5 μM of honokiol, the IC50 of CEM-C1 cells significantly decreased from 364.1 ± 29.5 to 126.2 ± 12.3 μM, and the reversal fold was 2.88, whereas the combination of dexamethasone and honokiol did not show significant impact on the IC50 of CEM-C7 cells as compared to that of CEM-C7 cells treated with dexamethasone alone (1.30 ± 0.13 versus 1.25 ± 0.14 μM; p > 0.05).


Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 24, 48, and 72 h-treatment by honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were cultured in DMEM supplemented with 10% of fetal bovine serum (FBS) at 37°C in a humidified atmosphere containing 5% of CO2.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were seeded at 3 × 105 cells/well in 6-well plates and pre-incubated for 24 h, and then treated with honokiol (0–20 μM) for 48 h. To extract the total protein, cells were washed with PBS, lysed in RIPA buffer for 30 min on ice.

Inhibition:

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: To further confirm whether honokiol increases the sensitivity of DEXresistant CEM-C1 cells to dexamethasone, the DEX- resistant CEM-C1 cells and DEX-sensitive CEM-C7 cells were incubated with different concentrations of dexamethasone (0–400 μM) in combination with honokiol (1.5 μM) for 48 h. Our preliminary drug concentration screening results showed that the cell proliferation inhibition rate on the CEM-C1 and CEM-C7 cells treated with 1.5 μM honokiol was <4% (Fig. S1).

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Different concentrations of dexamethasone (50, 100, 200, 300, and 400 μM) with or without 1.5 μM HNK were added to the medium of CEM-C1 cells which are dexamethasone-resistant, whereas dexamethasone of 0.5, 1, 2, 3, and 4 μM with or without 1.5 μM HNK were added to the medium of CEM-C7 cells which are dexamethasone-sensitive.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 48 htreatment by dexamethasone with or without 1.5 μM honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Upon treatment with dexamethasone in combination with 1.5 μM of honokiol, the IC50 of CEM-C1 cells significantly decreased from 364.1 ± 29.5 to 126.2 ± 12.3 μM, and the reversal fold was 2.88, whereas the combination of dexamethasone and honokiol did not show significant impact on the IC50 of CEM-C7 cells as compared to that of CEM-C7 cells treated with dexamethasone alone (1.30 ± 0.13 versus 1.25 ± 0.14 μM; p > 0.05).


Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 24, 48, and 72 h-treatment by honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were cultured in DMEM supplemented with 10% of fetal bovine serum (FBS) at 37°C in a humidified atmosphere containing 5% of CO2.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were seeded at 3 × 105 cells/well in 6-well plates and pre-incubated for 24 h, and then treated with honokiol (0–20 μM) for 48 h. To extract the total protein, cells were washed with PBS, lysed in RIPA buffer for 30 min on ice.

Flow Cytometry:

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: To further confirm whether honokiol increases the sensitivity of DEXresistant CEM-C1 cells to dexamethasone, the DEX- resistant CEM-C1 cells and DEX-sensitive CEM-C7 cells were incubated with different concentrations of dexamethasone (0–400 μM) in combination with honokiol (1.5 μM) for 48 h. Our preliminary drug concentration screening results showed that the cell proliferation inhibition rate on the CEM-C1 and CEM-C7 cells treated with 1.5 μM honokiol was <4% (Fig. S1).

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Different concentrations of dexamethasone (50, 100, 200, 300, and 400 μM) with or without 1.5 μM HNK were added to the medium of CEM-C1 cells which are dexamethasone-resistant, whereas dexamethasone of 0.5, 1, 2, 3, and 4 μM with or without 1.5 μM HNK were added to the medium of CEM-C7 cells which are dexamethasone-sensitive.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 48 htreatment by dexamethasone with or without 1.5 μM honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Upon treatment with dexamethasone in combination with 1.5 μM of honokiol, the IC50 of CEM-C1 cells significantly decreased from 364.1 ± 29.5 to 126.2 ± 12.3 μM, and the reversal fold was 2.88, whereas the combination of dexamethasone and honokiol did not show significant impact on the IC50 of CEM-C7 cells as compared to that of CEM-C7 cells treated with dexamethasone alone (1.30 ± 0.13 versus 1.25 ± 0.14 μM; p > 0.05).


Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 24, 48, and 72 h-treatment by honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were cultured in DMEM supplemented with 10% of fetal bovine serum (FBS) at 37°C in a humidified atmosphere containing 5% of CO2.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were seeded at 3 × 105 cells/well in 6-well plates and pre-incubated for 24 h, and then treated with honokiol (0–20 μM) for 48 h. To extract the total protein, cells were washed with PBS, lysed in RIPA buffer for 30 min on ice.

Transfection:

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: To further confirm whether honokiol increases the sensitivity of DEXresistant CEM-C1 cells to dexamethasone, the DEX- resistant CEM-C1 cells and DEX-sensitive CEM-C7 cells were incubated with different concentrations of dexamethasone (0–400 μM) in combination with honokiol (1.5 μM) for 48 h. Our preliminary drug concentration screening results showed that the cell proliferation inhibition rate on the CEM-C1 and CEM-C7 cells treated with 1.5 μM honokiol was <4% (Fig. S1).

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Different concentrations of dexamethasone (50, 100, 200, 300, and 400 μM) with or without 1.5 μM HNK were added to the medium of CEM-C1 cells which are dexamethasone-resistant, whereas dexamethasone of 0.5, 1, 2, 3, and 4 μM with or without 1.5 μM HNK were added to the medium of CEM-C7 cells which are dexamethasone-sensitive.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 48 htreatment by dexamethasone with or without 1.5 μM honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Upon treatment with dexamethasone in combination with 1.5 μM of honokiol, the IC50 of CEM-C1 cells significantly decreased from 364.1 ± 29.5 to 126.2 ± 12.3 μM, and the reversal fold was 2.88, whereas the combination of dexamethasone and honokiol did not show significant impact on the IC50 of CEM-C7 cells as compared to that of CEM-C7 cells treated with dexamethasone alone (1.30 ± 0.13 versus 1.25 ± 0.14 μM; p > 0.05).


Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 24, 48, and 72 h-treatment by honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were cultured in DMEM supplemented with 10% of fetal bovine serum (FBS) at 37°C in a humidified atmosphere containing 5% of CO2.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were seeded at 3 × 105 cells/well in 6-well plates and pre-incubated for 24 h, and then treated with honokiol (0–20 μM) for 48 h. To extract the total protein, cells were washed with PBS, lysed in RIPA buffer for 30 min on ice.

Knockdown:

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: To further confirm whether honokiol increases the sensitivity of DEXresistant CEM-C1 cells to dexamethasone, the DEX- resistant CEM-C1 cells and DEX-sensitive CEM-C7 cells were incubated with different concentrations of dexamethasone (0–400 μM) in combination with honokiol (1.5 μM) for 48 h. Our preliminary drug concentration screening results showed that the cell proliferation inhibition rate on the CEM-C1 and CEM-C7 cells treated with 1.5 μM honokiol was <4% (Fig. S1).

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Different concentrations of dexamethasone (50, 100, 200, 300, and 400 μM) with or without 1.5 μM HNK were added to the medium of CEM-C1 cells which are dexamethasone-resistant, whereas dexamethasone of 0.5, 1, 2, 3, and 4 μM with or without 1.5 μM HNK were added to the medium of CEM-C7 cells which are dexamethasone-sensitive.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 48 htreatment by dexamethasone with or without 1.5 μM honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: Upon treatment with dexamethasone in combination with 1.5 μM of honokiol, the IC50 of CEM-C1 cells significantly decreased from 364.1 ± 29.5 to 126.2 ± 12.3 μM, and the reversal fold was 2.88, whereas the combination of dexamethasone and honokiol did not show significant impact on the IC50 of CEM-C7 cells as compared to that of CEM-C7 cells treated with dexamethasone alone (1.30 ± 0.13 versus 1.25 ± 0.14 μM; p > 0.05).


Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: IC50 values in CEM-C1 and CEM-C7 cells after 24, 48, and 72 h-treatment by honokiol.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were cultured in DMEM supplemented with 10% of fetal bovine serum (FBS) at 37°C in a humidified atmosphere containing 5% of CO2.

Article Title: Honokiol induces apoptosis and autophagy in dexamethasone-resistant T-acute lymphoblastic leukemia CEM-C1 cells.
Article Snippet: CEM-C1 and CEM-C7 cells were seeded at 3 × 105 cells/well in 6-well plates and pre-incubated for 24 h, and then treated with honokiol (0–20 μM) for 48 h. To extract the total protein, cells were washed with PBS, lysed in RIPA buffer for 30 min on ice.



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